一種單克隆抗體能夠幫助鑒別人組織和血清中的glypican-3 (GPC3)。GPC3的表達(dá)是原發(fā)性肝癌的一個指標(biāo)。

GPC3屬于糖基磷脂酰肌醇-錨定細(xì)胞表面硫酸乙酰肝素蛋白多糖家族。1G12單克隆抗體已經(jīng)被用于惡性和非惡性肝臟組織中檢測GPC3表達(dá)，以及檢測血清中ELISA檢測GPC3。通過免疫組化檢測發(fā)現(xiàn)在蛋白水平，大多數(shù)原發(fā)性肝癌表達(dá)GPC3，包括小肝癌，但是在正常肝臟和良性肝臟病變檢測不出。在大部分原發(fā)性肝癌患者血清中GPC3水平顯著升高。這種單克隆抗體是BioMosaics公司(Burlington, VT, USA)的產(chǎn)品。
 
Monoclonal Antibody Helps Detect Liver Cancer
 
A monocloncal antibody is being used to aid the identification of glypican-3 (GPC3) in human tissues and serum. GPC3 expression is an indication of primary liver cancer.

GPC3 is a member of the glypican family of glycosyl phosphatidylinositol-anchored cell-surface heparan sulfate proteoglycans. The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and non-malignant liver tissue samples and for enzyme-linked immunosorbant assay (ELISA) detection of GPC3 in the serum. GPC3 is expressed at the protein level by immunohistochemistry in most cases of primary liver cancer including small tumors, but is undetectable in normal liver and benign hepatic lesions. GPC3 is significantly elevated in the serum of a large proportion of patients with primary liver cancer. The monoclonal antibody product was developed by BioMosaics (Burlington, VT, USA).