一系列即開(kāi)即用型實(shí)時(shí)聚合酶鏈反應(yīng)診斷試劑盒可以用于巨細(xì)胞病毒、乙肝病毒和EB病毒的DNA的定量檢測(cè)。所有試劑盒都有CE標(biāo)簽。

所有試劑盒只需要體積很小(200 μl)的樣本，具有敏感性高、動(dòng)力范圍廣泛、檢測(cè)協(xié)調(diào)性好，能夠使得不同分析所需的多次運(yùn)行在一次運(yùn)行中即可完成。這些試劑盒叫做affigenea trender，是由Sangtec分子生物學(xué)診斷試劑公司研制開(kāi)發(fā)的。用于檢測(cè)巨細(xì)胞病毒和EB病毒的試劑盒可以用于血漿、血清和全血，而檢測(cè)乙肝病毒的試劑盒只能用于血清和血漿。

Sangtec公司國(guó)際產(chǎn)品經(jīng)理Therese Sundell指出：“affigenea trender試劑盒，為不同樣本提供了靈活的相同的樣本處理方案，比如不含細(xì)胞的體液和全血。試劑盒還通過(guò)設(shè)立內(nèi)參照來(lái)防止出現(xiàn)假陰性的結(jié)果，通過(guò)UDG（尿嘧啶DNA糖基化酶）消毒以及封閉試管反應(yīng)來(lái)防止出現(xiàn)假陽(yáng)性結(jié)果。

Sangtec分子生物學(xué)診斷著眼于免疫減弱患者的感染性疾病和腫瘤學(xué)。該公司是世界上少數(shù)幾家獲得人類臨床診斷PCR執(zhí)照的公司之一。

Real-Time PCR Diagnostic Kits
 
A series of real-time polymerase chain reaction (PCR) diagnostic kits offer ready-to-use assays for DNA quantitation of cytomegalovirus (CMV), hepatitis B virus (HBV) and Epstein-Barr virus (EBV). All have the CE Mark.

All require only small sample volumes (200 μl) and are characterized by high sensitivity, wide dynamic range, and harmonized test profiles enabling multibatch runs with different analytes tested in one run. Called affigenea trender, the kits were developed by Sangtec Molecular Diagnostics (Stockholm, Sweden; www.sangtec.com). The assays for CMV and EBV are for use with plasma, scrum, and whole blood, while the HBV assay is for use in serum and plasma only.

“The affigenea trender kits offer a flexible solution for sample preparation from different sample matrices such as cell-free body fluids and whole blood,” noted Therese Sundell, international product manager at Sangtec. “They also offer prevention of false-negative results through a built-in internal control and prevention of false-positive results through UDG [uracil-DNA glycosylase] sterilization and closed-tube reactions.”

Sangtec Molecular Diagnostics focuses on infectious diseases of immunocompromised patients and oncology. The company states it is one of the few firms in the world with a broad PCR license for human clinical diagnostics.