一種新型血液RNA系統(tǒng)用于收集、存儲(chǔ)、運(yùn)輸血液，在密閉的試管中穩(wěn)定細(xì)胞內(nèi)RNA，以及后續(xù)的從全血中分離純化細(xì)胞內(nèi)RNA用于進(jìn)行分子診斷的逆轉(zhuǎn)錄PCR檢測(cè)。

PaxGene系統(tǒng)是由PreAnalytix GmbH公司 (Hombrechtikon, Switzerland)研制開發(fā)的。該系統(tǒng)已經(jīng)獲得了美國(guó)食品藥品管理局的許可并獲得了GE商標(biāo)，同時(shí)PreAnalytix得到了ISO 9001證書。

PaxGene血液RNA系統(tǒng)整合了全血收集、核酸穩(wěn)定以及RNA純化的關(guān)鍵步驟。通過將與RNA處理相關(guān)的不可預(yù)測(cè)性降低至最低，該系統(tǒng)提高了細(xì)胞內(nèi)RNA檢測(cè)的準(zhǔn)確性以及效率，并降低了成本。PaxGene系統(tǒng)包括血液收集管以及核酸處理試劑盒。收集試管包括了一種能夠使細(xì)胞內(nèi)RNA在室溫條件下穩(wěn)定3天、-20C條件下穩(wěn)定6個(gè)月的試劑。

Targeted診斷與治療公司的首席執(zhí)行官Daniel J.O’Shannessy博士說：“PaxGene血液RNA系統(tǒng)能夠顯著提高檢測(cè)信使RNA的能力。PaxGene系統(tǒng)提供的RNA穩(wěn)定性允許我們位于賓夕法尼亞州的實(shí)驗(yàn)室，從美國(guó)以及世界范圍內(nèi)接受血液樣本?！?/P>

Novel Blood RNA System
 
A new blood RNA system is for the collection, storage, transport of blood, the stabilization of intracellular RNA in a closed tube, and the subsequent isolation and purification of intracellular RNA from whole blood for reverse transcription polymerase chain reaction (RT-PCR) used in molecular diagnostic testing.

The PaxGene system was developed by PreAnalytix GmbH (Hombrechtikon, Switzerland). The system has been cleared by the U.S. Food and Drug Administration (FDA) and has received the CE Mark, while PreAnalytix has received ISO 9001 certification.

The PaxGene blood RNA system is an enabling technology that consolidates and integrates the key steps of whole blood collection, nucleic acid stabilization, and RNA purification. By minimizing the unpredictability associated with RNA processing, the system provides enhanced accuracy, greater efficiencies, and reduced costs of intracellular RNA analysis. Intracellular RNA is a key component of numerous diagnostic products. The PaxGene system comprises a blood collection tube and a nucleic acid processing kit. The collection tube contains a reagent that stabilizes intracellular RNA for three days at room temperature and up to six months at -20oC.

“The PaxGene Blood RNA system has significantly enhanced our ability to detect messenger RNA,” observed Daniel J. O’Shannessy, Ph.D., COO of Targeted Diagnostics & Therapeutics, Inc. (West Chester, PA, USA). “The stability of the RNA afforded by the PaxGene system allows us to received blood samples from across the United States and internationally into our laboratory in Pennsylvania.”